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Tube Guide &
Order of Draw
Order of draw
– in situations of multi-sampling, it is recommended to arrange the tubes in the following order.
Tube Volume
Determinations
Instructions
Green FA Plus
Blood culture bottle (410851)
Microbiology
To exclude the possibility of septicaemia
1 x set of blood culture bottles: both aerobic and anaerobic.
Using a butterfly scalp vein needle only add 8-10 mLs blood
to each bottle (do not overfill). Collect aerobic bottle first and
mix gently by inverting the bottles 8-10 times.
Storage and transportation:
room temperature
Paediatric bottles are available on request and must be
filled with 1-4 mLs of blood
Orange FN Plus
Blood culture bottle (410852)
Yellow PF Plus (Paed)
Blood culture bottle (410853)
Sodium Citrate
2.7mL
(363095)
Coagulation
PR,APTT, INR, Coagulation Studies (Note:
Coagulation Studies require 2 citrate tubes and
a 4mL EDTA tube)
Fill to at least Minimum Fill and not over Maximum Fill
Line marked on the tube
MIX WELL BY GENTLY INVERTING 8-10 TIMES
ACD
6.0mL
(368985)
Platelet estimation on patients with known
history of EDTA induced platelet clumping
MIX WELL BY GENTLY INVERTING 8-10 TIMES
Plain
10.0mL
(367895)
General
Biochemistry
Cardiac Markers,Troponin, U&E, LFT, Lipids,
HDL,Amylase, Lipase, Magnesium, Fe Studies,
Therapeutic Drugs, CRP,Vitamin D,Vitamin
B12, Glucose
Number of tubes required:
Up to 6 test groups – 1 tube e.g. LFT, Hepatitis,Amylase,
TFT, Hormones, Cardiac Enzymes
More than 6 test groups – 2 tubes
MIX WELL BY GENTLY INVERTING 8-10 TIMES
General Serology
General Serology, Hormones, Hepatitis Serology,
RAST, HIV
SST
8.5mL
(367958)
General
Biochemistry &
Serology
As above, however, limited therapeutic drug
levels – refer to specific details in the Pathology
Collection Guide for Doctors.
Lithium Heparin
6.0mL
(367885)
Biochemistry
Cholinesterase (Red Cell or Plasma),
Chromosome Analysis, Organophosphates
MIX WELL BY GENTLY INVERTING 8-10 TIMES
EDTA
4.0mL
(367839)
Haematology
FBC, Hb,WCC, Diff, Platelets, ESR, Malarial
Parasites, Retics
MIX WELL BY GENTLY INVERTING 8-10 TIMES
Biochemistry
Glycated Hb (HbA1c), Lead, FEP, G6PD, Porphryins
(Red Cell)
Molecular Genetics
EDTA acceptable, however separate red-rimmed
EDTA preferred
EDTA
6.0mL
(367941)
Blood Bank
Crossmatch, Group & Hold,Antibody Screen,
Blood Group
(Note: 6mL EDTA and 4mL EDTA tube required
for all Crossmatches and Group & Hold)
MIX WELL BY GENTLY INVERTING 8-10 TIMES
Note:
Full patient identification required for Crossmatching
and Group & Hold, Blood Group & Ab Screen
Doctor/Collector must initial tube to verify patient
details
Transfusion form required with any Crossmatch or Group
& Hold request
General Serology
PTH (additional plain or gel tube required
as well)
Red Rimmed
EDTA
4.0mL
(454003)
Flow Cytometry
Lymphocyte Surface Markers (additional 4mL
EDTA tube required for WCC), CD59, Paroxysmal
Nocturnal Haemoglobinuria
MIX WELL BY GENTLY INVERTING 8-10 TIMES
Molecular Genetics
(separate tube required)
Haemochromatosis, Factor V Leiden, MTHFR,
PGM, HLA B27, Fragile X, Coeliac Tissue Typing
Fluoride Oxalate
4.0mL
(367935)
Biochemistry
Glucose, Blood Alcohol (Medical)
MIX WELL BY GENTLY INVERTING 8-10 TIMES
Immunoserology
Tuberculosis
Collect 1mL of blood into each tube.
Shake tubes 10 times firmly enough to ensure inner
surface of tubes is coated. Do not spin and keep at room
temperature.
Quantiferon Gold Kit –
1.0mL each
Specimen Labelling – each tube must be legibly labelled with:
NOTE:All BD Vacutainer® tubes require
immediate mixing following collection
• Always fill tubes to full volume
• For any other tests please contact the laboratory
HAZARD WARNING: Never inject blood into the
tube from the needle and syringe.
- Surname
- First name
- Date of birth
- Gender (M or F)
- Date
- Time
- Collector’s initials
- Barcode